Table 1.
Proteins indentified in ventral disk proteomic of Giardia lamblia
| GEL1 | SPOT | NCBI2 | IDENTIFICATION | SCORE3 | PM (KDA)4 | PEPTIDES SEQUENCE5 |
|---|---|---|---|---|---|---|
| 1D | 1/3/4/5/6 | XP_001707372.1 | Beta tubulin [Giardia lamblia ATCC 50803] | 267 | 50701 | R.INVYFNEAAGGR.Y |
| R.FPGQLNADLRK.L | ||||||
| K.LAVNLIPFPR.L | ||||||
| R.VGEQFTAMFR.R | ||||||
| R.LHFFLVGFAPLTSR.G | ||||||
| K.GHYTEGAELVDAVLDVVRK.R | ||||||
| R.AILVDLEPGTMDSVR.A | ||||||
| K.MAATFIGNSTCIQELFKR.V | ||||||
| 1D | 1/2/3/4 | XP_001706843 | Alpha-tubulin [Giardia lamblia ATCC 50803] | 624 | 49617 | R.FDGALNVDLTEFQTNLVPYPR.I |
| R.AVFVDLEPTVVDEVR.A | ||||||
| R.QLYHPEQLISGKEDAANNYAR.G | ||||||
| R.NLDIERPTYTNLNR.L | ||||||
| R.LIAQCISSITASLR.F | ||||||
| K.TIGGGDDAFNTFFSETGAGK.H | ||||||
| R.GHYTIGKEIVDLVLDR.V | ||||||
| K.EIVDLVLDR.V | ||||||
| R.IHFPLCSYAPIISSEK.A | ||||||
| K.LSVAELTNSVFEPANMMVK.C | ||||||
| R.LIAQCISSITASLR.F | ||||||
| R.FDGALNVDLTEFQTNLVPYPR.I | ||||||
| R.IHFPLCSYAPIISSEK.A | ||||||
| K.LSVAELTNSVFEPANMMVK.C | ||||||
| K.VGINYQPPTVIPGGDLAK.V | ||||||
| 1D | 3/10 | XP_001706274.1 | Gamma giardin [Giardia lamblia ATCC 50803] | 543 | 35667 | K.MFQDHMVNDFRPK.F |
| 2D | 13 | R.HGLQTEINSLEAIIER.E | ||||
| R.LNQEVSNFKESFDASER.N | ||||||
| K.SSFSTVSSYSDHK.F | ||||||
| K.DAPTTIRDSLR.L | ||||||
| R.LTNAVEDPGYMTLSLISENKVDDLMFNSK.M | ||||||
| R.ELESTDLRSEVAK.Q | ||||||
| R.IFDAIMTTK.V | ||||||
| K.AFDAENADFVGK.T | ||||||
| 1D | 3 | XP_001707409.1 | Median body protein [Giardia lamblia ATCC 50803] 6 | 182 | 100579 | K.IDEYTLFMSR.S |
| 2D | 1 | R.ITELEQQQR.T | ||||
| K.VNNYDQLADDKAR.L | ||||||
| R.GNTSGVMAEIENIQR.Q | ||||||
| 1D | 4 | XP_001705181.1 | Hypothetical protein GL50803_16267 [Giardia lamblia ATCC 50803] 6 | 50 | 63191 | K.LGELDDEALAIIR.A |
| 1D | 5/10/11 | XP_001705425.1 | Beta-giardin [Giardia lamblia ATCC 50803] | 582 | 31043 | R.TLTQTMDKPDDLTR.S |
| K.SADNMYLTIKEEIDTMAANFR.K | ||||||
| K.SLAEMGDTLNNVETNLQNQIAIHNDAIAALR.K | ||||||
| K.EALKSLNDLETGIATENAER.K | ||||||
| K.SLNDLETGIATENAER.K | ||||||
| K.MYDQLNEKVAEGFAR.I | ||||||
| R.ISAAIEKETIAR.E | ||||||
| R.AVSAATTEALTNTKLVEK.C | ||||||
| R.AIQEEIDREK.A | ||||||
| 1D | 5 | XP_001704148.1 | Hypothetical protein GL50803_13584 [Giardia lamblia ATCC 50803]6 | 61 | 43838 | R.ATLAMELSNALDR.I R.IAELESILEGR.A |
| 1D | 11 | XP_001708306.1 | SALP-1 [Giardia lamblia ATCC 50803] | 122 | 29866 | R.YQHLYDAVNEQVLRR.Q |
| 2D | 8 | R.AIEEERAEFTENAGMLTR.E | ||||
| R.ELETVMKYLER.N | ||||||
| R.AYHSVIASYKDEIK.S | ||||||
| R.ETIVAGFSDLNR.A | ||||||
| 2D | 9 | ABV91519.1 | Mp1p-like protein 10 [Penicillium marneffei]6 | 79 | 15571 | -.VIEALLQFTNDIEKQSFQVLHK.D |
| K.DVVVILQR.I | ||||||
| R.IAVESQLDSR.D | ||||||
| R.IAVESQLDSRDVYSLEEGFK.A | ||||||
| R.DVYSLEEGFK.A | ||||||
| 2D | 9 | XP_001032825.1 | Hypothetical protein TTHERM_00486130 [Tetrahymena thermophila SB210]6 | 63 | 18838 | K.DAGRQISILVLGK.M |
| R.QISILVLGK.M | ||||||
| R.QISILVLGKMPEEFFDIGK.K | ||||||
| 2D | 10 | AAK32143.1 | giardin delta chain [Giardia intestinalis] | 147 | 30969 | R.VTDFHEDFKR.Q |
| K.IAQEHDDLLESIR.Y | ||||||
| K.TSAEESFGAFIGNLTNER.N | ||||||
| R.ADREQSIDEYLR.D | ||||||
| K.VLAGVVAELIATR.K | ||||||
Table with isolated ventral disc proteins of Giardia lamblia. The peptides identified were hydrolyzed with trypsin and submitted to Q-TOF and MALDI-TOF-TOF, like described in methodology
1- Gel utilized in mass spectrometry
2- National Center for Biotechnology Information: number of identification bank
3- Ideal value above 35 to efficient identification
4- Molecular weight (MASCOT)
5- Peptides to analysis based
6- These proteins were only identified in this paper and differ from other studies