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. 2012 Jan 19;5:41. doi: 10.1186/1756-0500-5-41

Table 1.

Proteins indentified in ventral disk proteomic of Giardia lamblia

GEL1 SPOT NCBI2 IDENTIFICATION SCORE3 PM (KDA)4 PEPTIDES SEQUENCE5
1D 1/3/4/5/6 XP_001707372.1 Beta tubulin [Giardia lamblia ATCC 50803] 267 50701 R.INVYFNEAAGGR.Y
R.FPGQLNADLRK.L
K.LAVNLIPFPR.L
R.VGEQFTAMFR.R
R.LHFFLVGFAPLTSR.G
K.GHYTEGAELVDAVLDVVRK.R
R.AILVDLEPGTMDSVR.A
K.MAATFIGNSTCIQELFKR.V

1D 1/2/3/4 XP_001706843 Alpha-tubulin [Giardia lamblia ATCC 50803] 624 49617 R.FDGALNVDLTEFQTNLVPYPR.I
R.AVFVDLEPTVVDEVR.A
R.QLYHPEQLISGKEDAANNYAR.G
R.NLDIERPTYTNLNR.L
R.LIAQCISSITASLR.F
K.TIGGGDDAFNTFFSETGAGK.H
R.GHYTIGKEIVDLVLDR.V
K.EIVDLVLDR.V
R.IHFPLCSYAPIISSEK.A
K.LSVAELTNSVFEPANMMVK.C
R.LIAQCISSITASLR.F
R.FDGALNVDLTEFQTNLVPYPR.I
R.IHFPLCSYAPIISSEK.A
K.LSVAELTNSVFEPANMMVK.C
K.VGINYQPPTVIPGGDLAK.V

1D 3/10 XP_001706274.1 Gamma giardin [Giardia lamblia ATCC 50803] 543 35667 K.MFQDHMVNDFRPK.F
2D 13 R.HGLQTEINSLEAIIER.E
R.LNQEVSNFKESFDASER.N
K.SSFSTVSSYSDHK.F
K.DAPTTIRDSLR.L
R.LTNAVEDPGYMTLSLISENKVDDLMFNSK.M
R.ELESTDLRSEVAK.Q
R.IFDAIMTTK.V
K.AFDAENADFVGK.T

1D 3 XP_001707409.1 Median body protein [Giardia lamblia ATCC 50803] 6 182 100579 K.IDEYTLFMSR.S
2D 1 R.ITELEQQQR.T
K.VNNYDQLADDKAR.L
R.GNTSGVMAEIENIQR.Q

1D 4 XP_001705181.1 Hypothetical protein GL50803_16267 [Giardia lamblia ATCC 50803] 6 50 63191 K.LGELDDEALAIIR.A

1D 5/10/11 XP_001705425.1 Beta-giardin [Giardia lamblia ATCC 50803] 582 31043 R.TLTQTMDKPDDLTR.S
K.SADNMYLTIKEEIDTMAANFR.K
K.SLAEMGDTLNNVETNLQNQIAIHNDAIAALR.K
K.EALKSLNDLETGIATENAER.K
K.SLNDLETGIATENAER.K
K.MYDQLNEKVAEGFAR.I
R.ISAAIEKETIAR.E
R.AVSAATTEALTNTKLVEK.C
R.AIQEEIDREK.A

1D 5 XP_001704148.1 Hypothetical protein GL50803_13584 [Giardia lamblia ATCC 50803]6 61 43838 R.ATLAMELSNALDR.I
R.IAELESILEGR.A

1D 11 XP_001708306.1 SALP-1 [Giardia lamblia ATCC 50803] 122 29866 R.YQHLYDAVNEQVLRR.Q
2D 8 R.AIEEERAEFTENAGMLTR.E
R.ELETVMKYLER.N
R.AYHSVIASYKDEIK.S
R.ETIVAGFSDLNR.A

2D 9 ABV91519.1 Mp1p-like protein 10 [Penicillium marneffei]6 79 15571 -.VIEALLQFTNDIEKQSFQVLHK.D
K.DVVVILQR.I
R.IAVESQLDSR.D
R.IAVESQLDSRDVYSLEEGFK.A
R.DVYSLEEGFK.A

2D 9 XP_001032825.1 Hypothetical protein TTHERM_00486130 [Tetrahymena thermophila SB210]6 63 18838 K.DAGRQISILVLGK.M
R.QISILVLGK.M
R.QISILVLGKMPEEFFDIGK.K

2D 10 AAK32143.1 giardin delta chain [Giardia intestinalis] 147 30969 R.VTDFHEDFKR.Q
K.IAQEHDDLLESIR.Y
K.TSAEESFGAFIGNLTNER.N
R.ADREQSIDEYLR.D
K.VLAGVVAELIATR.K

Table with isolated ventral disc proteins of Giardia lamblia. The peptides identified were hydrolyzed with trypsin and submitted to Q-TOF and MALDI-TOF-TOF, like described in methodology

1- Gel utilized in mass spectrometry

2- National Center for Biotechnology Information: number of identification bank

3- Ideal value above 35 to efficient identification

4- Molecular weight (MASCOT)

5- Peptides to analysis based

6- These proteins were only identified in this paper and differ from other studies