Figure 5.
Neutralization of rVSV-ZEBOVGP. Either wild-type VSV or recombinant VSV with its envelope G protein replaced with EBOV GP1,2 [rVSV-ZEBOVGP] was pre-incubated with the pooled sera, as indicated from each group at the final concentration of 1:25 dilution in the presence (A and C) or absence (B and D) of complement. Vero cells were exposed to the virus/serum mixture, and the virus titer was quantified by counting the number of plaques. The data shown represent analyses of pooled sera collected from mice in each group at week 40: VLP (n = 5), DNA (n = 6), DNA/VLP (n = 7), or serum from single animals for each of the following groups, PBS, CpG, and CpG/PBS. E, the data represent analysis of the sera collected from the indicated individual mice at week 37 prior to the last boosting. Shown here are the average titers +/- standard deviation (SD) of the triplicate samples tested. The titer was normalized to the inoculation titer, which was calculated from transduction in the absence of any mouse serum. Representative data from two or more experiments are shown.