Figure 6.

MxA relocalization in the presence of Ara-C. HeLa cells on coverslips were pre-treated for 24 h with 2000 U/ml of IFN-β. After pre-treatment, the cells were infected with MPXV-GFP-tdTR at an MOI = 1 in the presence 2000 U/ml of IFN-β and in the presence or absence of Ara-C. Coverslips were harvested, fixed, permeabilized, and immunostained for MxA 24 h p.i.. The coverslips were stained with Hoechst dye, mounted onto slides, and imaged by confocal microscopy. Green is GFP, red is Tomato Red, blue is Hoechst dye, and white is MxA.