Figure 11.

Centrosome association of Thr668 P-APP in mitotic cells: Asynchronously growing H4-APP cells were fixed and immunostained using Thr668 P-APP polyclonal and α-tubulin monoclonal antibodies and visualized using Alexa 594 (red) and 488 (green) fluorophores respectively. Nuclei were visualized using Hoechst staining. Staining was analyzed using the FV10-ASW 1.7 software for Olympus confocal microscope. Cells in metaphase and anaphase showed very clear P-APP localization at the centrosomes. Staining was very weak or absent in the interphase cells. Magnification: 63×.