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. 2012 Feb 22;7(2):e32062. doi: 10.1371/journal.pone.0032062

Figure 4. Mitochondria and calcium homeostasis are important for irradiated cells to change their autofluorescence.

Figure 4

A) Overlay histogram comparing background FL-1 fluorescence in untreated U87 and U87Rho(0) cells. U87 and mitochondria-deficient U87Rho(0) were treated with 10 Gy and FACS-analyzed 24 h later for B) FL-1 autofluorescence and C) cell size. D) Radiation dose-dependent changes in FL-1 autofluorescence in irradiated DC2.4 after 0, 2, 5, and 10 Gy correlate with changes in forward scatter (top) and side scatter (bottom). E) DC2.4 cells were irradiated with 0 or 10 Gy in the presence of 1 µM A23187 or 0.1% DMSO or 5%FBS/PBS and analyzed 24 h later for viability by 7-AAD exclusion (top). Viable cells were compared for cell size (bottom) and FL-1 autofluorescence (middle). Data are mean ± s.e.m. of n = 6 *p<0.05.