Figure 6. Inhibition of Gβγ-signaling prevents upregulation of PDE activity in lungs from OVA-sensitized/challenged rabbits and passively sensitized ASM tissues.

Relative to controls, PDE activity is significantly increased in lung tissues isolated from OVA-sensitized+challenged rabbits (A) and in OVA serum-sensitized (B) and IL-13-treated (C) isolated ASM tissues. The upregulated PDE activity is abrogated in lungs isolated from OVA-sensitized rabbits that are treated with inhaled anti-Gβγ peptide prior to antigen challenge, as well as in OVA serum- or IL-13-exposed ASM tissues that were pretreated with anti-Gβγ peptide or gallein. Data are mean ± SE values from 3–5 determinations. Comparisons are made using two-tailed Student t-test. **p<0.01.