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. 2012 Feb 22;7(2):e32078. doi: 10.1371/journal.pone.0032078

Figure 8. IL-13-induced Gi-βγ signaling mediates ERK1/2-dependent upregulation of PDE4D mRNA transcripts in HASM cells.

Figure 8

(A) IL-13 elicits temporal increases in PDE4D mRNA expression in HASM cells, with peak induction of transcripts detected at 12 hr. A representative experiment (B), and corresponding densitometric analysis of PDE4D mRNA expression expressed as a ratio of β-actin (C), demonstrate that IL-13 induced PDE4D transcripts at 12 hr is abrogated in HASM cells that are pretreated either with PTX, anti-Gβγ blocking peptide, or inhibitors of either MEK-ERK1/2 (U0126), c-Src tyrosine kinase (SU6656) or gallein, whereas pretreatment with inhibitors of p38 MAPK (SB202190) or JNK (SP600125) has no effect. Data are mean ± SE values from 4 experiments (**p<0.01).