Figure 3. c-Jun and c-Fos DNA binding in WT and Syk^−/− macrophages stimulated with mmLDL.

BMDM from WT or Syk^−/− mice (A and B) and J774 expressing control of Syk-specific shRNA (C and D) were incubated for 1 hour with media or 50 µg/ml mmLDL. Nuclear extracts were isolated and used in a transcription factor DNA binding plate-based assay. Mean ± SEM (n = 4). *, p<0.05; **, p<0.005 WT vs. Syk^−/−.