Fig. 2.

PI3K-dependent Akt-phosphorylation mediated by midazolam in B35 neuroblastoma cells. (A) The upper panel shows that midazolam increases levels of phosphorylated-Akt protein in a concentration-dependent manner. After treatment with the indicated concentrations of midazolam for 8 h, western blotting was performed. (B) The upper panel shows that midazolam induced phospho-Akt protein expression was diminished by LY-294002, an inhibitor of PI3 kinase. The indicated concentrations of the PI3 kinase inhibitor, LY-294002, were added 30 min prior to midazolam, and western blotting was performed after an 8 h incubation.