Figure 3.

Intrahepatic cholestasis of FXR^−/− mice after CCl₄ treatment. A, Hepatic BAs were measured as described in Materials and Methods. *, FXR^−/−vs. wild type (WT), P < 0.05; #, hepatic BAs of FXR^−/− mice treated with CCl₄vs. hepatic BAs of FXR^−/− mice at zero time. B–H, Quantitative real-time PCR analysis of CYP7A, BSEP, NTCP, OATP1, and MRP2/3/4 expression. The quantity of mRNA was normalized using the internal standard, m36B4. *, P < 0.05.