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. 2012 Jan 25;13:43. doi: 10.1186/1471-2164-13-43

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Copyright ©2012 Tu et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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NGS of the small RNA transcriptome. A1. Scatter plots of raw miRNA expression values of the same sample with the worst correlation. The x-axis was determined without barcode and the y-axis determined with barcode pair A4. A2. Scatter plots of raw miRNA expression values of the same sample with the best correlation. The x-axis was determined without barcode and the y-axis determined with barcode pair C3. Raw expression values of miRNAs determined with 16 kinds of barcode pairs were compared with raw expression values of miRNAs without barcode one by one, and the scatter plots are exhibited in Additional file 4. B. Read length distribution (nt) of known miRNAs by removing adaptors and barcodes. The y-axis depicts the percentage of read lengths relative to the total number of reads in all datasets. Read length distributions of each patient are shown in Additional file 2. C. Distribution of non-coding RNA species in the all 32 samples.