Table 1.

Multiplication ratios of E-MSCs and BM-MSCs on various culture systems. Multiplication ratios of E-MSCs and percentage of cells in S-phase at day 5 of a 7 day run, under various modes of culture

MSC and MC screening
BM-MSCs	Culture system	Multiplication ratio
	T-Flasks	0.4 ± 0.2
	Cultispher-S	0.16 ± 0.1
	Cytodex-3	-0.1 ± 0.12
	Cytopore-2	-0.5 ± 0.04
E-MSCs	T-Flasks	2.4 ± 0.1
	Cultispher-S	2 ± 0.3
	Cytodex-3	0.7 ± 0.6
	Cytopore-2	-0.6 ± 0.3
Maximization of MSC proliferation
E-MSCs on Cultispher-S	Mode of culture	Multiplication ratio	% of cells in S-phase at day 5
	Batch	1.5 ± 0.3	1.4 ± 0.3
	Cyclic-fed-batch	2.6 ± 0.2	8 ± 1
	Pulsed culture	3 ± 0.04	15 ± 1