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ELISA analysis of specificity of various subclonal antibodies. The specificity of antibodies produced by two hybridoma cell lines (B and E2) subcloned from the parental hybridoma cell line 6C9 were assessed using competitive ELISA analysis. Xanthine and 6-oxo-M1dG were used as competing antigens. The displayed results represent the average of spectroscopic readings of subclones E2 and B at 15 and 30 min postaddition of ABTS substrate.
