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. 2012 Feb 22;7(2):e31456. doi: 10.1371/journal.pone.0031456

Figure 1. Analysis of recombinant SjTGR-pET41a by enzyme restriction.

Figure 1

(A) Agarose gel (1%) electrophoresis of recombinant SjTGR-pET41a or pET41a vectors. Lane 1: Purified PCR amplified SjTGR gene fragment of about 1,800 bp. Lane 2: Products of recombinant plasmid SjTGR-pET41a digested by Nde I/Sal I. The sizes of the pET41a backbone and the SjTGR gene were about 5,000 bp and 1,800 bp, respectively. Lane 3: Products of plasmid pET 41a digested by Nde I/Sal I. The sizes of the fragments from Nde I/Sal I digestion of pET41a were about 5,000 bp and 900 bp. Lane 4: Undigested pET 41a plasmid of 5933 bp. Lane 5: Undigested recombinant plasmid SjTGR-pET41a of about 6,800 bp. Note that both undigested plasmids migrated lower in the gel due to the super-helical structures. MW: DNA molecular weight marker. (B) Schematic diagram of the SjTGR-pET41a plasmid showing relevant enzyme sites and the SjTGR insert size.