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. 2012 Feb 22;7(2):e31456. doi: 10.1371/journal.pone.0031456

Figure 2. Electrophoretic patterns of expression products of recombinant SjTGR.

Figure 2

(A) Expression products from E. coli BL21 transformed with recombinant SjTGR-pET41a and induced with 1 mmole/L of IPTG at 37°C for 4 h. Lane 1: Supernatant of bacterial lysate. Lane 2: Precipitate of bacteria lysate resuspended with PBS. The black arrow indicates the expressed SjTGR protein band. MW: Protein molecular weight marker. (B) Expression products from transformed or non-transformed E. coli BL21 induced with 0.5 mmole/L of IPTG at 24°C for 24 h. Products in precipitates (lane 1) or supernatant (lane 2) of induced bacteria containing plasmid SjTGR-pET41a show expression of the recombinant SjTGR (black arrow). Expression products of induced bacteria containing plasmid pET41a (lane 3) or of induced non-transformed bacteria (lane 4) are also shown. MW: Protein molecular weight marker.