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. 2012 Feb 22;7(2):e31951. doi: 10.1371/journal.pone.0031951

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Thomas et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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¹H NMR analysis demonstrated differences in the composition of HILIC-HPLC fractions from L. reuteri 6475. A. Top and bottom spectra show the 1D ¹H NMR of TNF-inhibitory fraction B3 and fraction B4, respectively. The assigned peaks of phenylalanine (Phe), tryptophan (Trp), and histamine from fraction B3 are labeled on the top spectrum. Two complementary 2D NMR techniques were used to identify compounds unique to TNF-inhibitory fraction B3. B. Aromatic peaks of ¹H-¹³C-HSQC from Phe, Trp, and histamine. C. Aliphatic peaks of ¹H-¹³C-HSQC from Phe, Trp, and histamine. D. The TOCSY spectrum further identified the spin system of Phe, Trp, and histamine unique to TNF-inhibitory fraction B3.