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. 2012 Feb 22;7(2):e31855. doi: 10.1371/journal.pone.0031855

Figure 5. Influence of the mutation in fbaB on the expression of hrp-hrc-hpa genes of X. oryzae pv. oryzicola.

Figure 5

(A) Semi-quantitative RT-PCR analysis. RNAs were isolated from cultures of the wild-type RS105 strain and the fbaB mutant RΔfbaB grown in rice suspension cells for 16 h. The 16S rRNA gene of the pathogen is used as the standard internal control. The tested hrp-hrc-hpa genes were selected based on the reports [19], [20], [53] with the primer pairs (Table S1) and the sequence of the hrp clusters (AF272885, AY875714) was used as the reference. (B) Real-time quantitative RT-PCR analysis. The relative mRNA level of the tested hrp-hrc-hpa genes in the fbaB mutant RΔfbaB was calculated with respect to the level of the corresponding transcripts in the wild-type RS105 cultured in rice suspension cells for 16 h. Values given are the means ± SD of triplicate measurements from a representative experiment. The asterisks in each horizontal data column indicate significant differences. **, P = 0.01, t test. Experiment was repeated twice and yielded similar results.