Table 4. Phage displayed peptide sequences binding to myeloma immunoglobulins.* .
| MM | Lib. X7 | Lib. X12 | Lib. X18 | Lib. BS |
| 001† | YPNYEKWSRWPF | GLPIASPEKYTFWPWKLW | ||
| SNMTLKELMWPF | TFVGAYRTQWLEQGRFPH | |||
| 003† | VWYAWMEDLDHAPEFWKI | |||
| 008† | RTPIPKPAWSWRSLAYNS | |||
| PQDFYRSRFWDAHWMYWQ | ||||
| PRDYRSLLYGFHDLTTKV | ||||
| 020† | YKTPYHMWQEWV | WYYPDEEAYENFYELWSR | ||
| AQHNFIETSYDMFQQFRF | ||||
| 021‡ | DRDIYQLSNLVEYLA | FLNGCDKEDWMCWVTT | ||
| GSGYSSNNLVAQKPKATL | ||||
| 022‡ | TDPWSKS | WHICLDERAMCQIKELRV | KSYGCKEERAHCYGQV | |
| WAPFSLL | QDQYTDCSIKGEHTMVCG | IRMQCREERSSCSMIS | ||
| GTWVCRDERATCRSAS | ||||
| SKVVCYDERCTCGRNA | ||||
| MSGRCLEHRATCYPWN | ||||
| 023‡ | VIGPSSL | TQARLDWVNDEYAPSLR | PELRCLEWIHICYAIG | |
| YFSPSFH | GILLRSFQEFWLNEEYMY | GRNICLLGDHQCAQVN | ||
| RWENDEFWGMISLKEAML | ||||
| YRWLMRVGIQDRHHQEMG | ||||
| 025‡ | PDLSSQRYIIEGSGQKPQ | SLSPCQVWDAGCQSAG | ||
| PILQYPLVTGTGTKEGIH | REEVCMETVPICDLYF | |||
| PLGQSGRDKAESKTKGWT | GKHLCRPWGLACVAMQ | |||
| PKILSGAGNKVEGASHGA | ||||
| GRAGEKMISGDGNKIHES | ||||
| RDQGYRAPYHVGLGARSG | ||||
| 026‡ | PEQYCCRSRWSCQICH | |||
| SREVVWWDGLSLVRSR | ||||
| LLTPCVTRWGNCMQGL | ||||
| TPSHCPSIRARCQATI | ||||
| HPYLCAMRSDRCAMTV | ||||
| AQFLCSDLGRVCTRVS | ||||
| 031‡ | SEAIQKNSGNEQ | |||
| FGQQKTSGNEEG | ||||
| NGLLHQKNAGNE | ||||
| ALHKTSGNEMDI | ||||
| AGHPKDSGNEWA | ||||
| 034‡ | ADSSKRCNFNQC | |||
| YNGTPSTVHLLP | ||||
| 036‡ | EPYTPHLGQALW | |||
| IXXRGLPTDMXG | ||||
| GFESSGKYLRKQ | ||||
| VDFPNAWKWWTE | ||||
| SSAGPEQPRLMW | ||||
| YPELPRWFPPNY | ||||
| VPQLPRYYSVSS | ||||
| QIPGWLRTGLEA |
*Libraries with 7-mer linear (X7), 12-mer linear (X12), 18-mer linear (X18), and 14-mer constrained beta sheet (BS) inserts were selected on myeloma Ig of patients MM001–MM036. The table lists the peptide insert sequences (single letter amino acid code) of phage clones for which specific binding to the respective Fab fragment and/or paraprotein was validated in single clone binding assays.
†
phage selection on monoclonal Fab fragment.
‡
phage selection on paraprotein.