Figure 3. Loss of spore OD₆₀₀ of 11 C. difficile BI/NAP1/027 isolates during incubation in A) BHIS supplemented with taurocholate and chenodeoxycholate; B) BHIS supplemented with taurocholate; or C) BHIS alone.

A), B), and C) ▴, DH1834; ▾, DH1432; ▪, 26173; •, 26131; ♦, DH478; ▵, 05-1223-046; □, DH361; ○, DH1858; ◊, DH1466; ▿, CDC38; and <$>\raster="rg4"<$>, 5108111. A) The dotted line represents the loss of OD₆₀₀ of the positive control strain (05-1223-046) in response to BHIS supplemented with 0.1% (w/v) taurocholate alone. B) The dotted line represents the negative control strain (DH361) during incubation with both 0.1% (w/v) taurocholate and 2 mM chenodeoxycholate (a strain found, in preliminary studies, not to germinate when exposed to chenodeoxycholate). C) The dotted line represents the loss of OD₆₀₀ of the positive control strain (05-1223-046) in response to BHIS supplemented with 0.1% (w/v) taurocholate alone. All data represent the average of three independent experiments and error bars indicate the standard errors of the means.