Table 2. Percentage loss of spore optical density after incubation of C. difficile spores with bile salts for 2 h.
| Strain name | PCR-ribotype | 0.1% taurocholate | 2 mM chenodeoxycholate | BHIS |
| 9001966 | 002 | 47% (0.529) | 3% (0.968) | 11% (0.894) |
| 8085054 | 014 | 72% (0.376) | 13% (0.868) | 38% (0.623) |
| 5108111 | 027 | 53% (0.473) | 40% (0.599) | 27% (0.725) |
| CDC 38 | 027 | 42% (0.580) | 12% (0.888) | 6% (0.938) |
| DH1432 | 027 | 49% (0.508) | 0% (1.031) | 15% (0.846) |
| DH1834 | 027 | 58% (0.420) | 57% (0.429) | 27% (0.734) |
| DH1466 | 027 | 64% (0.355) | 0% (1.079) | 15% (0.852) |
| DH1858 | 027 | 70% (0.301) | 9% (0.910) | 23% (0.768) |
| DH478 | 027 | 52% (0.479) | 0% (1.113) | 5% (0.947) |
| DH361 | 027 | 58% (0.422) | 5% (0.947) | 9% (0.911) |
| 26131 | 027 | 50% (0.500) | 0% (1.036) | 7% (0.934) |
| 26173 | 027 | 46% (0.537) | 5% (0.946) | 4% (0.961) |
| 05-1223-046 | 027 | 74% (0.257) | 0% (1.009) | 38% (0.623) |
| 7004578 | 078 | 46% (0.536) | 36% (0.644) | 7% (0.926) |
| CD 2315 | 078 | 47% (0.534) | 33% (0.671) | 21% (0.792) |
C. difficile spores were heat treated at 60°C for 25 min and then incubated with either i) BHIS supplemented with 0.1% (w/v) taurocholate; ii) BHIS supplemented with 0.1% (w/v) taurocholate and 2 mM chenodeoxycholate; or iii) BHIS alone. The data represent the average of three independent experiment and numbers in parentheses represent the mean of the final OD600 value.