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. Author manuscript; available in PMC: 2012 Feb 23.
Published in final edited form as: J Thromb Haemost. 2010 Dec;8(12):2751–2756. doi: 10.1111/j.1538-7836.2010.04077.x

Fig. 2.

Fig. 2

Reversible green fluorescent protein (GFP) reporter expression in the megakaryocyte/platelet lineage. (A) Flow cytometry analysis of GFP expression in CD41+ platelets in peripheral blood of representative wild type (left panel) and CMV-rtTA; TRE-GFP-shLuc bi-transgenic (right panel) mice after doxycycline treatment of indicated durations. Percent GFP+ cells in bi-transgenic samples are indicated. (B) Flow cytometry analysis of GFP expression in peripheral blood platelets of representative CMV-rtTA; TRE-GFP-shLuc mice after 1 week on doxycycline (green) followed by a week off doxycycline (black), showing reversible GFP expression in bi-transgenic platelets. PercentGFP+cells are indicated. (C) Flow cytometry analysis of doxycycline-induced GFP expression in Lin– Sca1–cKit+CD150+CD41+ megakaryocyte precursors isolated from bone marrow of wild type (black) and CMV-rtTA; TRE-GFP-shLuc bi-transgenic (green) mice. Percent GFP+ cells from bi-transgenic mice is indicated.