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. 2011 Oct 17;2(12):730–742. doi: 10.1021/cn200090z

Figure 8.

Figure 8

Dose–response curves for ML218 (26b). (A) Structure of ML218 (26b). (B) Raw fluorescence-based HTS assay traces. (C) CRC from fluorescence-based assay (IC50 = 150 nM). (D) Inhibition time-course of ML218. Ba2+ currents were elicited by pulse depolarized to −20 mV from a holding potential of −90 mV (200 ms, 0.2 Hz). The bath application of ML218 caused rapid inhibition and slow partially recovery after washout (n = 5). (E) Cav3.2 IonWorks Quattro (patch EP) CRC IC50 = 310 ± 15 nM. (F) Cav3.3 IonWorks Quattro (patch EP) CRC IC50 = 274 ± 53 nM.