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. 2012 Jan 9;287(8):5192–5198. doi: 10.1074/jbc.C111.331686

FIGURE 2.

FIGURE 2.

Functional significance of galectin-3 cleavage by PSA. A, the effect of recombinant full-length galectin-3 and 1–107 and 108–250 fragments on endothelial cell morphogenesis. Three-dimensional heterotypic co-cultures of BAMEC and LNCaP cells on Matrigel were performed in the presence of 10 μg/ml recombinant galectin-3 and its fragments. B, quantitative evaluation of tube formation assay. C, chemotaxis assay in LNCaP cells. Full-length galectin-3 (10 μg/ml) increases chemotaxis in LNCaP cells as compared with fragments of galectin-3 and control cells. Data points show the mean ± S.E. (n = 3) in each condition. D, prostate cancer cells cell migration in the scratch assay. The assay was performed with LNCaP, Du145, and PC3M cell lines. The scratch assay was done to assess in vitro migration of cultured prostate cancer cells stimulated by full-length galectin-3, galectin-3 1–107, and galectin-3 108–250. A uniform wound was made in each plate using a 1-μl pipette tip. The wound area was observed immediately, and at 24 h after creation, cells were counted. Cells were grown under identical conditions. E, a pathway graphic describing the possible mechanisms for the roles of PSA-resistant galectin-3 in the tumorigenesis and progression of prostate cancer.