FIGURE 2.

Cat.G induces FAK ubiquitination. A–C, NRCMs were pretreated with MG132, lactacystin or their vehicle and treated with Cat.G for the indicated time (A and C) or for 2 h (B). Left: lysates were immunoprecipitated with anti-FAK antibodies and blotted with anti-ubiquitin (Ub), -phosphotyrosine (P-Tyr), -ubiquitin-Lys⁴⁸ (Ub-Lys⁴⁸), or -FAK antibodies. Right: Quantification of experiments expressed as mean ± S.E. from three separate cultures. *, p < 0.05 versus control, #,p < 0.05 versus Cat.G-treated cells.