FIGURE 5.

c-Cbl mediates FAK ubiquitination and degradation induced by Cat.G. A, neonatal myocytes derived from WT or c-Cbl KO mice were treated with Cat.G for 2 h and were assessed for immunoblot analysis. B, NRCMs were infected with WT-Cbl or C381A-Cbl at the indicated pfu/cell and were assessed for c-Cbl immunoblot. C and D, NRCMs infected with adenoviruses expressing Lac-Z (10 pfu/cell), C381A-Cbl (5 pfu/cell) or WT-Cbl (10 pfu/cell) were treated with Cat.G for 2 h and assessed for immunoprecipitation assay or immunoblot analysis. Left: representative immunoblots with GAPDH taken as loading control. Right: quantification of experiments expressed as mean ± S.E. from three separate cultures. *, p < 0.05 versus control, #, p < 0.05 versus Cat.G-treated cells.