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Determination of disulfide linkages in SepCysS. A, disulfide content in A. fulgidus SepCysS was determined with the alkaline sulfide/cyanolysis assay. B, free thiol content in M. jannaschii SepCysS was determined with DTNB titration. + indicates that the Cys residue is intact; − indicates that the Cys residue is altered to Ala. Error bars represent S.D. from three independent assays.
