FIGURE 1.
Decorin inhibits VEGFA-mediated angiogenesis by attenuating pro-angiogenic factors. A, RT2 PCR Profiler Angiogenesis Array evaluating pro-angiogenic gene expression changes in HeLa cells following exposure to 500 nm decorin after 24 h. B, PCR array target gene verification performed via qPCR under the same conditions as in A. PCR Profiler Angiogenesis Array and all subsequent qPCR verification analyses were performed in triplicate among three independent experiments, normalized to the endogenous housekeeping gene, ACTB, and reported as average fold-changes ± S.E. in agreement with the comparative ΔΔCt method (please see ”Experimental Procedures“ for a full and detailed description).