FIGURE 6.

Heparin-induced leukocytosis depends on blockade of CXCL12 function. The kinetics of leukocytosis (A) and the levels of CXCL12α (B) and CXCL12β (C) in peripheral blood and in BM after heparin administration. The data were summarized from three experiments with 10 mice per group. D, blocking CXCL12 function with neutralizing antibody attenuated HIL response. The mice were preinjected i.v. with neutralizing anti-CXCL12 antibody prior to heparin or saline injection and then analyzed for HIL. The data were summarized from three experiments with 7–9 mice per group. Each bar represents the average value ± S.E. The statistical analysis was carried out by paired Student's t test. WBC, white blood cells. Ab, antibody.