Effect of FTY720 on the expression of cyclin Ds and cyclin-dependent kinase (CDK) inhibitors in db/db islets.
A, the mRNA levels of cyclin-dependent kinase inhibitors. *, p < 0.01. B, the mRNA levels of cyclin Ds. *, p < 0.01. Total RNAs were extracted from islets isolated from control and FTY720-treated mice at the age of 13 weeks, and the mRNA levels of interested targets were analyzed by RT-PCR. C, cyclin D3 immunostaining of pancreas after 6 weeks FTY720 treatment. The cells in the islet with cyclin D3-staining in their nucleus (brown) are indicated by black arrows (scale bar, 50 μm).