FIGURE 8.
Effect of FTY720 on p57KIP2 and cyclin D3 expression in the wild type islets. A, Western blot analysis. Islets were isolated from 8-week-old wild type C57/BL6 mice and treated with 0.1 or 0.2 μm FTY720 in cell culture medium without FBS. After 24 and 48 h, protein was extracted and analyzed by Western blot analysis. B, quantitation of cyclin D3 mRNA and C, p57KIP2 mRNA levels. Isolated islets were treated with 0 (control), 0.1 or 0.2 μm FTY720 or FTY720-P for 48 h, and total RNAs were extracted and quantitative real-time RT-PCR was performed. *, p < 0.001, versus control.