TABLE 1.
Sequences of primers and probes used for conventional and real-time RT-PCRs
| Gene | Sequences |
|---|---|
| Primers for conventional RT-PCRs (mouse) | |
| GAPDH | F: 5′-AACTTTGGCATTGTGGAAGGGCTC |
| R: 5′-ACCCTGTTGCTGTAGCCGTATTCA | |
| Cyclin D1 | F: 5′-TGCTGCAAATGGAACTGCTTCTGG |
| R: 5′-TACCATGGAGGGTGGGTTGGAAAT | |
| Cyclin D2 | F: 5′-AGGCCCAAGTTGAATGAGTCTGGA |
| R: 5′-ATCAGGTACCCAAGTTGCCCAAGA | |
| Cyclin D3 | F: 5′-TCCCACAGATGTCACAGCCATTCA |
| R: 5′-AAGCTGGTTGAGTGGGAAGGAAGA | |
| p21CIP1 | F: 5′-AATCCTGGTGATGTCCGACCTGTT |
| R: 5′-AGACCAATCTGCGCTTGGAGTGAT | |
| p27KIP1 | F: 5′-TGGCTCTGCTCCATTTGACTGTCT |
| R: 5′-ATGATTGACCGGGCCGAAGAGATT | |
| p57KIP2 | F: 5′-TGTACCATGTGCAAGGAGTACGCT |
| R: 5′-TTGGTATGGGCAGTACAGGAACCA | |
| S1P1 | F: 5′-TTCTCTTCTGCACCACCGTCTTCA |
| R: 5′-AGCAGGCAATGAAGACACTCAGGA | |
| S1P3 | F: 5′-TGGCTTCTCCATTTCCTCCTGTGT |
| R: 5′-ACGGATCCACTGGGCATACATCAA | |
| S1P4 | F: 5′-ATCTAGTGCTTGCCTCCCATCCAA |
| R: 5′-GGCCTGGGTTTCCTGTTGTTTGAA | |
| S1P5 | F: 5′-TTTCCAATAGCCGCTCTCCTGTGT |
| R: 5′-AGCTTGCCGGTGTAGTTGTAGTGA | |
| PI3Kr1 | F: 5′-CAGCAACCGAAACAAAGCGGAGAA |
| R: 5′-TGGCTTGAAGGTGAGGAACTGGAT | |
| PI3Kr2 | F: 5′-ATCAAAGTCTTCCACCGGGATGGT |
| R: 5′-TGAGGTCAGGTTTGAGGCTGTTCA | |
| PI3Kr3 | F: 5′-TGCTTTGGAAAGAAGCTGGCTTGG |
| R: 5′-AGGGCAAACAGGGTGATGAAGAGA | |
| Akt1 | F: 5′-ACGGGCACATCAAGATAACGGACT |
| R: 5′-AAGGTGGGCTCAGCTTCTTCTCAT | |
| Akt2 | F: 5′-TGGACTGCTGAAGAAGGACCCAAA |
| R: 5′-TTTCTGTACCACGTCCTGCCAGTT | |
| Primers and probes for quantitative real-time RT-PCRs (mouse) | |
| GAPDH | F: 5′-TCAACAGCAACTCCCACTCTTCCA |
| R: 5′-ACCCTGTTGCTGTAGCCGTATTCA | |
| Probe-/56-FAM/GGCTGGCAT/Zen/TGCTCTCAATGACAACT/3IABKFQ | |
| Cyclin D3 | F: 5′-TCT TCC TTC CCA CTC AAC CAG CTT |
| R: 5′-TTT GGC AAC TGA GAA GGT TGG AGC | |
| Probe-/56-FAM/TCCTGGGCC/Zen/ATGATGGTCAGAGAAAT/3IABKFQ | |
| p57KIP2 | F: 5′-ATG TAG CAG GAA CCG GAG ATG GTT |
| R: 5′-TTT ACA CCT TGG GAC CAG CGT ACT | |
| Probe-/56-FAM/TGAGAACAC/Zen/TCTGTACCATGTGCAAGG/3IABKFQ | |
| PI3Kr1 | F: 5′-AAT AGG TTA CAG TGC GGG CCG TAT |
| R: 5′-CAG TTT CCT TGG CTT TGC TCG GTT | |
| Probe-/56-FAM/CCAAGACAC/Zen/CATTACAAAGAAAGCCGGAC/3IABkFQ | |
| PI3Kr2 | F: 5′-TGC ATC CAG CAA GAT CCA AGG AGA |
| R: 5′-ACC ATC CCG GTG GAA GAC TTT GAT | |
| Probe-/56-FAM/TCAGGAAAG/Zen/GCGGGAACAACAAGTTG/3IABkFQ | |
| PI3Kr3 | F: 5′-TTC AGA CAT TGC TGT GCG GTT GTG |
| R: 5′-GCA AGT CTG CCA ACC ATT CCA AGT | |
| Probe-/56-FAM/GGGAAGGTA/Zen/AGGTGAGAACATTGTTGGG/3IABkFQ | |
| PDX-1 | F: 5′-ACT TAA CCT AGG CGT CGC ACA AGA |
| R: 5′-GGC ATC AGA AGC AGC CTC AAA GTT | |
| Probe-/56-FAM/AATTCTTGA/Zen/GGGCACGAGAGCCAGTT/3IABkFQ | |
| Bcl-2 | F: 5′-AAT TGT AAT TCA TCT GCC GCC GCC |
| R: 5′-ACA CTC CGG CTT CAC TGA GAA TGT | |
| Probe-/56-FAM/TGCGGTGCT/Zen/CTTGAGATCTCTGGTT/3IABkFQ | |
| Bcl-xL | F: 5′-TTG CTG GTC GCC GGA GAT AGA TTT |
| R: 5′-TGG GTC TGC TCT GTG TTT AGC GAT | |
| Probe-/56-FAM/ACTTATCTT/Zen/GGCTTTGGATCCTGGAAGAGA/3IABkFQ | |
| Primers and probes for quantitative real-time RT-PCRs (rat) | |
| GAPDH | F: 5′-TGA TGC TGG TGC TGA GTA TGT CGT |
| R: 5′-TCT CGT GGT TCA CAC CCA TCA CAA | |
| Probe-/56-FAM/AGTCTACTG/Zen/GCGTCTTCACCACCAT/3IABkFQ | |
| Cyclin D3 | F: 5′-TCA CTG CAT TTG GAT CTG GGT CCT |
| R: 5′-AGT TTA CGG GCG CTT GCT CTT CTA | |
| Probe-/56-FAM/AG CCA ACC T/Zen/A GAT GGC TGC TGT GTA A/3IABkFQ | |
| p57KIP2 | F: 5′-TCG AAT TCG CCC TTA GCG ATG GAA |
| R: 5′-GCA CAT CCT GCT GGA AGT TGA AGT | |
| Probe-/56-FAM/TC CAG CGA C/Zen/A CCT TCC CAG TGA TA/3IABkFQ | |
| PDX-1 | F: 5′-AGT TGG GTA TAC CAG CGA GAT GCT |
| R: 5′-TTG TCC TCA GTT GGG AGC CTG ATT | |
| Probe-/56-FAM/TC TGG GAC T/Zen/C TTT CCT GGG ACC AAT T/3IABkFQ | |