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. 2011 Dec 19;287(8):5615–5626. doi: 10.1074/jbc.M111.314088

FIGURE 3.

FIGURE 3.

MTA1 negatively regulates RNF144A expression at mRNA level. A and B, Western blot analysis of the protein extracts from MCF-7 cells stably expressing pcDNA empty vector (MCF-7/pcDNA) and T7-MTA1 (MCF-7/T7-MTA1) with the indicated antibodies (A) and quantitative results of Western blots (B) using the ImageJ software. C, HeLa cells were transfected with control siRNAs or specific siRNAs targeting human MTA1. After 48 h of the second-round transfection, protein extracts were prepared and subjected to Western blot analysis with the indicated antibodies. Vinculin was shown as a loading control. D, quantitative results of Western blots shown in C using the ImageJ software. E and F, qRT-PCR analysis of the expression of RNF144A and MTA1 mRNA levels in the MCF-7/pcDNA and MCF-7/T7-MTA1 stable clone cells (E) and Hela cells transfected with control siRNAs or specific siRNAs targeting human MTA1 (F). G, MCF-7 cells were transfected with control siRNAs or specific siRNAs targeting human MTA1. After 36 h of the second-round transfection, cells were treated with or without 250 ng/ml of Actinomycin-D (Act-D) for another 12 h, and then subjected to qRT-PCR analysis of the expression of RNF144A and MTA1 mRNA levels as described above.