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. 2011 Dec 19;287(8):5615–5626. doi: 10.1074/jbc.M111.314088

FIGURE 4.

FIGURE 4.

MTA1 is recruited onto human RNF144A promoter and inhibits RNF144A promoter activity. A, line diagram showing the regions of human RNF144A promoter analyzed. Nomenclature uses first and last nucleotide number, with the translational start being +1. R, region. B and C, ChIP analysis for the recruitment of MTA1 (A) or HDAC2 (B) onto human RNF144A promoter in MCF-7 cells. D, double ChIP analysis for the recruitment of the MTA1/HDAC2 complex onto human RNF144A promoter. The first ChIP with an anti-MTA1 antibody was followed by the second ChIP with an anti-HDAC2 antibody. E and F, MTA1+/+ and MTA1−/− MEFs (E) or MCF-7/pcDNA and MCF-7/T7-MTA1 stable clone cells (F) were transfected with human RNF144A luciferase reporter plasmid. After 36–48 h of transfection, RNF144A luciferase activity was determined as described under “Experimental Procedures.”