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. 2011 Dec 19;287(8):5615–5626. doi: 10.1074/jbc.M111.314088

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — A, EMSA analysis of the binding of c/EBPα and MTA1 to human RNF144A promoter using nuclear extract from the MCF-7 cells. B, nuclear extracts from MCF-7 cells were subjected to immunoprecipitation (IP) analysis with an anti-MTA1 (lane 3), anti-c/EBPα (lane 4) antibody or control IgG (lane 2), followed by Western blotting with the indicated antibodies. WB, Western blotting. C, EMSA analysis of the binding of MTA1 and c/EBPα to the oligos with wild-type or mutant c/EBPα binding motif on human RNF144A promoter. D, MCF-7 cells were transfected with a pGL3-RNF144A luciferase reporter plasmid along with the indicated expression vectors, and RNF144A luciferase activity was determined as described above.