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. 2011 Dec 19;287(8):5615–5626. doi: 10.1074/jbc.M111.314088

FIGURE 7.

FIGURE 7.

RNF144A is a novel inhibitor of migration and invasion and the effect of MTA1 on tumor migration and invasion is dependent on RNF144A expression. A and B, effect of depletion of endogenous RNF144A by specific RNF144A siRNAs on cell migration capacity. MCF-7/pcDNA and MCF-7/T7-MTA1 stable clone cells were transfected with control siRNAs or RNF144A siRNAs and then subjected to wounding-healing assay as described under “Experimental Procedures.” The representative images of migrated cells after 24 h and the quantification of the average difference in wound closure between 0 and 24 h are shown in A and B, respectively. C and D, effect of depletion of endogenous RNF144A by specific RNF144A siRNAs on cell invasive capacity. MCF-7/pcDNA and MCF-7/T7-MTA1 stable clone cells were transfected with control siRNAs or RNF144A siRNAs and subjected to Boyden chamber invasion assay. The representative images of cells that invaded the Matrigel Invasion chambers (scale bars, 0.5 mm) and the quantification of the total number of invaded cells are shown in C and D, respectively. Error bars indicate S.D. E, an abstract model for the role of the MTA1-RNF144A pathway in cell migration and invasion.