CXCR2, NHERF1, and PLC-β2 form a macromolecular complex in vitro and in neutrophils.
A, schematic representation of in vitro macromolecular complex assembly (upper panel, refer to “Experimental Procedures” for details). Macromolecular complex of PLC-β2 C-tail, PDZ scaffold proteins, and human full-length CXCR2 (lower panel) are shown. B, macromolecular complex of PLC-β2 full-length, PDZ scaffold proteins, and mouse CXCR2 C-tail. C, dose-dependent (GST-NHERF1) macromolecular complex formation of His-S-tagged PLC-β2 C-tail, GST-NHERF1, and HA-tagged human CXCR2. D, endogenous PLC-β2 and NHERF1 were co-precipitated with CXCR2 from murine bone marrow neutrophils.