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. 2011 Dec 27;287(8):5744–5755. doi: 10.1074/jbc.M111.315762

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Disrupting the CXCR2 macromolecular complex inhibits CXCR2 ligand-induced neutrophil transepithelial migration. A, IL-8-induced dHL-60 cell transepithelial migration across HT-29 cell monolayers. dHL-60 cells (3 × 10⁶) were pre-delivered with various human CXCR2 C-tail peptides as indicated, and the cells that migrated into the lower chamber at 2 h were quantified by a colorimetric MPO assay. **, p < 0.01 compared with control (no peptide). B, transepithelial migration of dHL-60 cells across HT-29 monolayers induced by fMLP. dHL-60 cells (3 × 10⁶) were pre-delivered with various human CXCR2 C-tail peptides as indicated, and the transmigration was quantified by MPO activity assay.