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. 2011 Dec 28;287(8):5819–5832. doi: 10.1074/jbc.M111.295964

FIGURE 1.

FIGURE 1.

Effect of STAT3 on expression of epithelial and mesenchymal markers in LoVo and SW1116 cells. A representative Western blot (A) and the summarized data (B) show that knockdown of STAT3 expression significantly decreased the phosphorylation of STAT3 and the expressions of STAT3, N-cadherin, and vimentin in LoVo cells, whereas E-cadherin expression was dramatically increased, indicating that STAT3 may participate in the regulation of epithelial and mesenchymal markers in colon cancer. Another representative Western blot (C) and the summarized data (D) show that STAT3 overexpression significantly increased the phosphorylation of STAT3 and expressions of STAT3, N-cadherin, and vimentin in SW1116 cells, whereas E-cadherin expression was dramatically reduced. Seventy-five pmol of siRNA duplexes of STAT3, control siRNA, or plasmids complexed with liposomes were applied to each well. After 72 h of transfection, the cells were collected for analysis. n = 3, t test; *, p < 0.01; **, p < 0.05, compared with the pCDNA3.1 or control siRNA groups. Error bars, S.E.