FIGURE 1.
Assembly and purification of the complete BLOC-1 complex. A, schematic of the polycistronic expression cassettes used for the expression and purification of recombinant BLOC-1 in pST39. B, Coomassie Blue gel of BLOC-1 consisting of pallidin, dysbindin(1–217,) BLOS2, Cappuccino, Snapin, BLOS3, BLOS1, and Muted subunits. C, analytical ultracentrifugation of the full BLOC-1 complex at 5.3 μm. D, a lysate of human MNT1 and mouse melan-a melanoma cells was applied to GST, GST-AP-2 core, and GST-BLOC-1 immobilized on glutathione-Sepharose. After washing, the bound proteins were analyzed by SDS-PAGE and immunoblotting using the antibodies indicated. 2% of the input is shown.