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. 2011 Dec 27;287(8):5917–5927. doi: 10.1074/jbc.M111.295733

FIGURE 2.

FIGURE 2.

Effects of protein silencing on tube formation on EA.hy926 cells. A, 48 h after transfection with siRNA (against STIM1, Orai1, TRPC3, TRPC4, and TRPC5), the EA.hy926 cells were seeded on growth factor-reduced Matrigel and visualized 10–14 h later. B, tube formation was quantified in control condition and upon siRNA treatment. Data are mean ± S.E. (error bars) of three to five independent experiments. *, p < 0.05; ***, p < 0.001. C, cells were seeded on growth factor-reduced Matrigel in control conditions and in the presence of 10 μm TRPC3 inhibitor Pyr3 or 2 μm phospholipase C inhibitor U73122. Scale bars, 200 μm.