FIGURE 4.

Spontaneous Ca²⁺ oscillations on EA.hy926 cells during tube formation. A–D, cytosolic Ca²⁺ measured at 37 °C on EA.hy926 cells loaded with 2 μm Fura-2 (supplemental Movies 1 and 2). Intracellular Ca²⁺ variations were measured after the first 1–2 h of tube formation on Matrigel, after siControl treatment (A), siSTIM1 (B), siTRPC3 (C) or siTRPC4 (D). Acquisition was done for 10 min, and each panel displays the responses of a representative coverslip. E, number of Ca²⁺ oscillations during 10 min in cells treated with siControl, siSTIM1, siOrai1, siTRPC3, siTRPC4, and siTRPC5. F, percentage of cells showing at least one Ca²⁺ transient during the 10-min recording time (n ranges from 64 to 168 cells). Error bars, S.E. ns, not significant; **, p < 0.01; ***, p < 0.001.