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. 2011 Dec 27;287(8):5917–5927. doi: 10.1074/jbc.M111.295733

FIGURE 7.

FIGURE 7.

Expression of TRPC isoforms on HUVEC and effects of protein silencing on tube formation. A, mRNA amplification of TRPC1, TRPC3, TRPC4, TRPC5, TRPC6, and TRPC7 isoforms by real-time PCR in HUVECs. B, mRNA levels assessed by quantitative RT-PCR 48 h after transfection (mean ± S.E. (error bars), 2–4 independent experiments). C, 48 h after transfection with siRNA (against TRPC1, TRPC4, STIM1, and Orai1), the HUVECs were seeded on growth factor-reduced Matrigel and visualized 10–14 h later. D, quantification of tube formation in control condition and upon siRNA treatment. Data are mean ± S.E. of three to five independent experiments. Scale bar, 200 μm. ns, not significant; ***, p < 0.001.