FIGURE 8.

Spontaneous Ca²⁺ oscillations during the tube formation on HUVECs. A and B, cytosolic Ca²⁺ measured at 37 °C on HUVECs loaded with 2 μm Fura-2. Intracellular Ca²⁺ variations were measured after the first 1–2 h of tube formation on Matrigel, after siControl treatment (A) and siTRPC4 (B). Acquisition was done for 10 min, and each panel displays the responses of a representative coverslip. C, percentage of cells showing at least one Ca²⁺ transient during the 10-min recording time (n ranges from 39 to 135 cells). D, number of Ca²⁺ oscillations during 10 min in cells treated with siControl, siTRPC1, siTRPC4, siSTIM1, and siOrai1. ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001.