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. 2011 Dec 20;287(8):6014–6024. doi: 10.1074/jbc.M111.316315

FIGURE 2.

FIGURE 2.

TRPV4 transcripts increased neurite outgrowth. A, NGF induced-neuritogenesis in PC12 cells and primary cultures of DRG neurons for 6 days. To better visualize neurites (green), cells were stained with anti-TuJ1 conjugated with Alexa Fluor® 488 goat anti-mouse IgG. Scale bar, 10 μm. B, increase of TRPV4 transcript levels in NGF-treated PC12 cells. mRNA levels were quantified by RT-PCR. Data were normalized versus GAPDH. C, increases in TRPV4 transcript levels in primary cultured DRG neurons. mRNA levels were quantified by RT-PCR. Data were normalized versus GAPDH.