FIGURE 3.

Effects of TRPV4 siRNA and TRPV4 overexpression on neurite outgrowth. A, TRPV4 mRNA levels were measured by RT-PCR in control and RNAi-treated groups in PC12 cells. GAPDH was used as a positive control. B and C, TRPV4 siRNA-transfected cells showed less neurite outgrowth and fewer cells with neurites than scrambled siRNA-treated PC12 cells. Scale bar, 10 μm. D, when treated with 5,6-EET, cells expressing TRPV4 (TRPV4^WT) showed an immediate increase in [Ca²⁺]_i. In contrast, a loss of function mutant of TRPV4 (TRPV4^P19S) evoked a markedly lower Ca²⁺ response to 5,6-EET. E, the overexpression of TRPV4^WT promoted neurite growth and increased the number of cells with neurites.