Table 4.
Colony formation by single primary MPNST cells cultured under non-adherent conditions and tumor formation by these colonies after transplantation in vivo
| Tumor | Single cells that formed colonies (%) | Tumors/colonies injected | Single cells that formed tumorigenic colonies (%) |
|---|---|---|---|
| Nf1+/−; Ink4a/Arf−/− mice | |||
| 6 | 26 | 2/9 (22%) | 5.8 |
| 7 | 47 | 4/5 (80%) | 38 |
| 8 | 39 | 2/7 (29%) | 11 |
| 9 | 14 | 5/11 (45%) | 6.2 |
| 10 | 22 | 5/10 (50%) | 11 |
| 11 | 34 | 8/13 (62%) | 21 |
| 12 | 66 | 12/13 (92%) | 60 |
| 14 | 11 | 4/5 (80%) | 8.7 |
| 15 | 7.6 | 2/7 (29%) | 2.2 |
| 16 | 38 | 9/11 (82%) | 31 |
| 17 | 66 | 9/10 (90%) | 59 |
| 23 | 55 | nd | nd |
| Mean±SD | 35±20 | 62/100 (62%) | 23±21 |
| Nf1+/−;p53+/− mice | |||
| 1 | 10 | nd | nd |
| 2 | 3.1 | nd | nd |
| 3 | 6.1 | 0/10 (0%) | 0.0 |
| 4 | 2.4 | 1/5 (20%) | 0.5 |
| 5 | 0.5 | nd | nd |
| 6 | 8.3 | 4/4 (100%) | 8.3 |
| 7 | 0.5 | nd | nd |
| 8 | 5.6 | 4/10 (40%) | 2.2 |
| 9 | 1.6 | nd | nd |
| 12 | 0.5 | 1/1 (100%) | 0.5 |
| 13 | 8.1 | 1/6 (17%) | 1.4 |
| 14 | 2.9 | 2/4 (50%) | 1.5 |
| Mean±SD | 4.1±3.4** | 13/40 (32%) | 2.0±2.9* |
For each tumor, we determined the percentage of cells that formed colonies in culture and the percentage of these colonies that formed tumors upon transplantation into C57BL/Ka mice. The last column represents the product of these statistics: the percentage of MPNST cells that formed tumorigenic colonies.
**
p=3×10−5;
*
p=0.02. All statistics represent mean ± SD. For data on cell death in culture see Figure S3.