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. Author manuscript; available in PMC: 2013 Feb 21.
Published in final edited form as: J Am Coll Cardiol. 2012 Feb 21;59(8):751–763. doi: 10.1016/j.jacc.2011.10.888

Figure 3. Histological and immunohistochemical assessment.

Figure 3

(a) H&E of matrix injected infarct demonstrating moderate mononuclear cell infiltration (scale bar = 50 μm). (b) An increased average area of cardiomyocyte islands were found in the infarct of myocardial matrix injected hearts compared to saline controls (*P<0.05). An island of viable myocardium in the infarct of a matrix injected heart is denoted by the arrow (scale bar = 200 μm). Cardiac Troponin T is shown in green with the infarct and borderzone denoted by I and BZ, respectively. (c) There was no significant difference in the number of infiltrating M2 macrophages between groups. Nuclei are stained blue with Hoechst and CD163+ cells are labeled red with a few cells denoted by the arrow points (scale bar = 100 μm). (d) An increase in proliferative cells were observed in matrix injected hearts (*P<0.05). Nuclei are stained blue with Hoechst and Ki67+ cells are labeled red. Co-stained nuclei appear purple with a couple cells denoted by arrow points (scale bar = 50 μm). (e) C-kit+ cells were observed in low numbers within the myocardial matrix scaffold. C-kit+ cells (denoted with arrow points) are labeled in green, with nuclei in blue (scale bar = 10 μm).