TABLE 1. List of antibodies evaluated.

Antibody	Antigen Target	Isotype	Concentration	Volume	Source
Monoclonal antibodies
PAC-1 fluorescein	Activated human GPIIbIIIa	IgM	25.0 μg/mL	20 μL	BD Biosciences (San Jose, CA, USA)
Antihuman CD109-PE	Human platelet actuation factor	IgG1	20.0 μg/mL	10 μL	Chemicon (Temecula, CA, USA)
Antihuman CD62P-PE clone # AK-4	Human CD62P	IgG1	*	20 μL	BD Biosciences
Antihuman CD63-fluorescein	Human CD63	IgG1	*	20 μL	BD Biosciences
BAQ 125A	Bovine activated platelet epitope	IgG1	15.0 μg/mL	5 μL	Washington State University Monoclonal Antibody Center (WSUMAC; Pullman, WA, USA)
BAQ 56A	Bovine activated platelet epitope	IgG1	15.0 μg/mL	5 μL	WSUMAC
GC5A	Bovine activated platelet epitope	IgG1	15.0 μg/mL	5 μL	WSUMAC
Antibovine CD63	Bovine CD63	IgG1	*	10 μL	Serotec (Raleigh, NC, USA)
CAPP2A	Ovine CD41/61	IgG1	7.5 μg/mL	5 μL	Veterinary Medical Research & Development (VMRD; Pullman, WA, USA)
GB20A	85 kD protein on bovine platelets	IgG1	7.5 μg/mL	5 μL	VMRD
GB84A	Bovine CD42d	IgG1	7.5 μg/mL	5 μL	WSUMAC
NPL44-10	Human CD62P	IgG1	25.0 μg/mL	5 μL	Takara Bio (Shiga, Japan)
MCA2419	Human CD62P	IgG1	25.0 μg/mL	5 μL	Serotec
MCA2420	Human CD62P	IgG1	25.0 μg/mL	5 μL	Serotec
6F3	Ovine CD62P	IgG1	25.0 μg/mL	5 μL	Harvard University (Cambridge, MA, USA)
Isotype control antibodies
IgM-fluorescein	IgM isotype control	IgM	25.0 μg/mL	20 μL	Southern Biotech (Birmingham, AL, USA)
Coli S69	IgG1 isotype control	IgG1	Matched for each IgG1 Ab experiment	—	WSUMAC
Rabbit IgG	Rabbit IgG isotype control	IgG	30.0 μg/mL	5 μL	Southern Biotech
Polyclonal antibody
Rabbit polyclonal antihuman CD62P	Human CD62P	N/A	30.0 μg/mL	5 μL	BD Biosciences

^*Concentrations not provided by manufacturer.

N/A, not applicable.