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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Mol Microbiol. 2011 Dec 7;83(1):179–194. doi: 10.1111/j.1365-2958.2011.07925.x

Figure 3. Transwell assay to determine if agr operon-mediated regulation of CPB production involves a diffusible signaling molecule.

Figure 3

In a single Transwell plate, two wells (top and bottom) are separated by a membrane filter impermeable to bacteria but having pores that allow diffusion of small molecules. Shown in the top panel is the inoculation strategy used in the experiment. The bottom panels show, respectively, results after the top well of each specified culture supernatant from a 5 h culture was subjected to CPB Western blotting. Shown in the Figure are representative results that were reproducible over three repetitions. The migration of purified CPB is indicated on the Western blot.