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. 2012 Feb 23;8(2):e1002503. doi: 10.1371/journal.pgen.1002503

Figure 6. Wif1 affects Hh activity in shf discs.

Figure 6

(A–C) Wild-type wing discs, showing the regions with high levels (red bars) of CiAct (A) or Ptc (B), or showing the ventral accumulation of Hh-GFP in the posterior compartment after dorsal, ap-Gal4-driven expression of UAS-hh-GFP (C). (D–F) shf/Y wing discs show reductions in the width of domains expressing high levels of CiAct (D) or Ptc (E), and also show reduced ventral accumulation of Hh-GFP in the posterior compartment after dorsal ap-Gal4-driven expression of UAS-hh-GFP (F). (G–I) shf/Y wing discs with ap-Gal4-driven expression of UAS-wif1 have a broader domain of less intense anti-CiAct staining (G) and lower levels of anti-Ptc staining (H), but improve the ventral accumulation of Hh-GFP in the posterior compartment after ap-Gal4-driven expression of UAS-hh-GFP (I). Ventral Hh-GFP was more punctuate than in wild type discs (compare to C). To make it easier to see the differences in ventral Hh-GFP accumulation, levels were increased equally in the boxed regions in C, F, and I. Hh-GFP levels are quantified in Figure S9.